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Data Acquisition - Scanners
•Make an image of the slide by
–Exciting the fluorescent dyes
–Collecting the emitted light
–Generating digital images of the fluorescent signal and
–Quantifying the intensity of the signal
•Two major types
–Laser excitation with a photomultiplier tube (PMT) detector - faster
–Filtered white-light excitation with a charge-coupled device (CCD) detector