Objectives for 3 Days
•To become familiar with the wet-bench basics of 2-color
DNA microarrays for
expression analysis
•In the context of 2-color expression arrays, use
GeneSpring for selection of
differentially expressed genes and for cluster analysis
•To identify points at which uncertainties and
inaccuracies may arise in
2-color expression analysis
•In the context of 2-color expression arrays, to use
EXCEL for processing of primary
data
•Optional
–To work with annotation of microarray data
(optional)
–To work with a major microarray data base
(optional)
–To become aware of several additional applications for
microarray technology
(optional)
•SNP analysis
•Transcription factor binding site analysis
•Protein analysis
•Cell and Tissue analysis